DNA-DEPENDENT RNA-DIRECTED PROTEIN SYNTHESIS in vitro , I. EXTENT OF GENOME TRANSCRIPTION
- 1 February 1969
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 62 (2) , 483-489
- https://doi.org/10.1073/pnas.62.2.483
Abstract
We have described an in vitro system which couples UTP incorporation into RNA by DNA-dependent RNA polymerase with RNA-directed amino acid incorporation into polypeptides. Using the technique of DNA-RNA hybridization, we have demonstrated that the whole ΦX-174 genome is transcribed during concomitant protein synthesis.Keywords
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