Further studies on the interaction between polynucleotides and antibodies to poly(inosinic acid)·poly(cytidylic acid)

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Abstract
Interactions between rabbit antibodies to poly(I) .cntdot. poly(C) (or Fab fragments) and double-stranded polynucleotides were studied by several techniques. In quantitative precipitation tests, the amounts of precipitated antibodies depend upon salt concentration. This effect of salt is reversible. From the comparison of the association constants (deduced from fluorescence measurements) for the binding of Fab fragments to several polynucleotides, a direct interaction between atoms or groups of the bases, which can be involved in H bonds, can be excluded. The complexes between poly(I) .cntdot. poly(C) and Fab fragments interact with ethidium bromide. The association constants for the binding of ethidium bromide to poly(I) .cntdot. poly(C) and to poly(I) .cntdot. poly(C)-Fab fragments complexes are about the same, but Fab fragments reduce the number of base pairs available to ethidium bromide binding. At saturation of poly(I) .cntdot. poly(C) by Fab fragments, the number of binding sites of ethidium bromide is only reduced by a factor of 2. Apparently all nucleotide residues covered by the Fab fragment binding site do not interact with the amino acid residues. Circular dichroism studies of the complexes between Fab fragments and polynucleotides are in favor of some conformational change of the polynucleotides.