Effect of hindlimb isolation procedure on isogravimetric capillary pressure and transcapillary fluid dynamics in dogs.

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Abstract
We measured isogravimetric capillary pressure (Pci) and plasma colloid osmotic pressure (pip) in isolated dog hindlimbs. A very rapid isolation technique and a perfusion technique involving no weight change were developed in order to compare the effects of different isolation procedures. Also, a "previous isolation procedure" (PIP) was used to approximate isolation procedures previously reported; this procedure included (A) anesthesia for 1 to 1 1/2 hours before limb isolation, (B) 1/2 hour of denervation before isolation, and (C) perfusion after isolation for 1/2 hour at an arterial pressure of 100 mm Hg and a venous pressure of 6 mm Hg. These different procedures altered average (+/- SE) capillary pressure and fluid dynamics in the hindlimb as shown in the foly pressure in the intact resting dog hindlimb may be 8 mm Hg below plasma colloid osmotic pressure, or about 9 mm Hg, and that many reported values have been heavily influenced by the isolation technique.