Stimulation of T-independent antibody responses by hapten-lipopolysaccharides without repeating polymeric structure

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Abstract
The murine immune response to a haptenated lipopolysaccharide (LPS) lacking repeating oligosaccharide determinants was studied. The LPS was extracted from a rough strain of bacteria (Salmonella minnesota R595) and chemically haptenated with trinitrophenol or fluorescein isothiocyanate. These preparations of hapten-R595 LPS were immunogenic. The immune response to the hapten was demonstrated to occur independent of T [thymus-derived] cells and was not merely the result of enhanced polyclonal B[bone marrow-derived]-cell activation. The capacity of such hapten-LPS conjugates without repeating polymeric structures to stimulate T cell-independent antibody responses provides information on the molecular requirements for the activation of murine B lymphocytes.