The Germination of Spores of Clostridium tetani
Open Access
- 1 April 1972
- journal article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 70 (2) , 253-261
- https://doi.org/10.1099/00221287-70-2-253
Abstract
SUMMARY: The spores of Clostridium tetani will germinate aerobically and anaerobically in complex media such as heart infusion broth or tryptone with yeast extract at 37° and pH 7.5. A synthetic medium as effective as heart infusion broth for germination under both aerobic and anaerobic conditions consists of 1 mM-methionine, 100 mM-lactate, 82 μM-nicotinamide, 280 mM-Na+ in 40 mM-phosphate buffer, pH 7.5. Anaerobic germination would take place in either methionine with Na+ or lactate and nicotinamide with Na+, but all the germinants were essential aerobically. Heat activation did not occur and thioglycollate increased the rate of germination.Keywords
This publication has 0 references indexed in Scilit: