SUBSTRATE AND NUTRIENT EFFECTS UPON EPIDERMAL BASAL CELL ORIENTATION AND PROLIFERATION
- 1 August 1964
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 52 (2) , 252-259
- https://doi.org/10.1073/pnas.52.2.252
Abstract
Basal cells of embryonic epidermis remain oriented and mitotically active if cultured in vitro on a suitable substrate and in the presence of chicken embryo juice or a macromolecular fraction therefrom. Millipore filters, tropocollagen gels, or frozen-thawed dermls are effective substrates, whereas lens paper or 100,000-g centrifugal pellets are not. The active fraction from embryo juice is non-dlalyzable, heat-labile, trypsin-sensitive, and sediments between 2000 and 100,000 g.Keywords
This publication has 6 references indexed in Scilit:
- DNA SYNTHESIS, MITOSIS, AND DIFFERENTIATION IN PANCREATIC ACINAR CELLS IN VITRO The Journal of cell biology, 1964
- THE INDUCTION OF DIFFERENTIATION AND THE RESPONSE TO THE INDUCER.1964
- Tissue Interactions in Scale and Feather Development as Studied in Dermal-Epidermal RecombinationsDevelopment, 1963
- Effects of extra-epithelial factors on the incorporation of thymidine by embryonic epidermisExperimental Cell Research, 1963
- The Stimulation of Epidermal Keratinization by a Protein Isolated from the Submaxillary Gland of the Mouse**From the Department of Biochemistry and the Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee.Journal of Investigative Dermatology, 1963
- Tissue interactions during skin histodifferentiationDevelopmental Biology, 1962