Guanine nucleotides and magnesium dependence of the association states of the subunits of transducin
- 10 December 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 178 (2) , 228-232
- https://doi.org/10.1016/0014-5793(84)80606-7
Abstract
When GTPγS is bound to transducin (T), the two subunits Tα·GTPγS and Tβγ dissociate, independently of the ionic environment. When GDP is bound, these subunits are associated as a monomeric Tα·GDP‐Tβγ complex of 75 kDa when the ionic environment is comparable to that of the cytoplasm, but they dissociate in the presence of 10–100 mM Mg2+ or Ca2+ Using this property, the subunits could be separated and purified by a rapid one‐step procedure on an ion‐exchange column (FPLC), and their molecular masses were verified by neutron small angle scattering. The physiological relevances of the dissociating effect of Mg2+ are discussed.Keywords
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