Analytical Fractionation of Homogenates from Ehrlich Ascites Tumor Cells
- 1 January 1979
- journal article
- research article
- Published by Wiley in Bulletin des Sociétés Chimiques Belges
- Vol. 88 (1-2) , 99-105
- https://doi.org/10.1002/bscb.19790880115
Abstract
Homogenates of Ehrlich Ascites tumor cells have been assayed for cytochrome c oxidase, β‐N‐acetylglucosaminidase, acid deoxyribonuclease, α‐L‐fucosidase, inosine diphosphatase, NADH and NADPH cytochrome c reductase, phosphoglucomutase, RNA and UNA. The optimal conditions for each enzyme assay have been established. The subcellular fractions have been obtained by differential centrifugation and identified by their specific enzyme markers.Keywords
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