Further studies on erythrocyte inorganic pyrophosphatase: an examination of different mammalian species and human‐Chinese hamster hybrid cells

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Abstract
SUMMARY: Erythrocyte inorganic pyrophosphatase has been examined electrophoretically in 32 different species of mammals. The electrophoretic mobilities have been compared and secondary isozyme production has been seen to be notably more marked in certain species, particularly the higher primates.Special attention has been given to the enzyme in the mouse and Chinese hamster. In cultured cells, the mouse enzyme is indistinguishable from that of the human. The Chinese hamster enzyme moves more anodally than the human enzyme but its pattern is somewhat confused by other pyrophosphatases.A human‐hamster hybrid clone was examined. It was found to have a human erythrocyte inorganic pyrophosphatase complement. A single hybrid band intermediate between the human and hamster bands was seen. This was taken as further evidence that the enzyme is dimeric.