Guanine nucleotides can activate the insulin‐stimulated phosphodiesterase in liver plasma membranes

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Abstract
The insulin‐stimulated cyclic AMP phosphodiesterase from liver plasma membranes is shown to be activated upon incubation with guanine nucleotides in the presence of ATP. The non‐hydrolysable analogue of ATP, adenylyl imidodiphosphate failed to substitute for ATP in achieving activation. GTP, its non‐hydrolysable analogues p[NH]ppG and GTP‐γ‐S, as well as GDP, all elicited activation. It is suggested that guanine nucleotides, and probably insulin, exert their effect on this enzyme through a distinct species of guanine nucleotide regulatory protein.