Mithramycin and DIPI: A pair of fluorochromes specific for GC- and AT-rich DNA respectively
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 36 (3) , 299-305
- https://doi.org/10.1007/bf00446280
Abstract
The AT specificity of the fluorochromes DIPI and DAPI and the GC specificity of mithramycin are evidenced by observations in human, mouse, and bovine chromosomes. DIPI and DAPI produce a pattern similar to Hoechst 33258 in all three species, whereas mithramycin results in a reverse pattern. The AT-rich centromeric heterochromatin in mouse is brilliantly stained by DIPI or DAPI and remains nearly invisible after mithramycin staining. In the GC-rich centromeric heterochromatin of cattle the opposite behavior is observed.This publication has 38 references indexed in Scilit:
- DAPI fluorescence of plant chromosomes prestained with actinomycin DExperimental Cell Research, 1976
- The use of antinucleoside antibodies to probe the organization of chromosomes denatured by ultraviolet irradiationExperimental Cell Research, 1974
- Centromeric heterochromatin and comparison of G-banding in cattle, goat, and sheep chromosomes (Bovidae)Cytogenetic and Genome Research, 1974
- Multiple satellite deoxyribonucleic acids in the calf and their relation to the sex chromosomesJournal of Molecular Biology, 1973
- Why Centric Regions of Quinacrine-treated Mouse Chromosomes show Diminished FluorescenceNature, 1973
- An analysis of the bovine genome by Cs2SO4—Ag+ density gradient centrifugationJournal of Molecular Biology, 1973
- Staining of constitutive heterochromatin in mammalian chromosomes with a new fluorochromeExperimental Cell Research, 1972
- Satellite DNA in calf heterochromatinExperimental Cell Research, 1971
- Density gradient analysis of newly replicated DNA from synchronized mouse lymphoma cellsExperimental Cell Research, 1971
- How Different are the DNAs from Related Animals?Nature, 1968