Crystallographic Studies on a Family B DNA Polymerase from Hyperthermophilic Archaeon Pyrococcus kodakaraensis Strain KODI
- 1 June 1999
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 125 (6) , 983-986
- https://doi.org/10.1093/oxfordjournals.jbchem.a022405
Abstract
A hyperthermostable family B DNA polymerase from the hyperthermophilic archaeon, Pyrococcus kodakaraensis strain KOD1, has been crystallized by the hanging-drop vapor diffusion method at 293 K with 2-methyl-2,4-pentanediol as the precipitant. The diffraction pattern of a crystal extends to 3.0 Å resolution, and two full sets of 3.0 Å resolution diffraction data for native crystals were successfully collected at 290 K and 100 K upon exposure to synchrotron radiation at KEK-PF, Japan. The crystals belong to the space group, P212121, with unit-cell dimensions of a=112.8, b=115.4, and c=75.4 Å at 290 K, and a=111.9, b=112.4, and c=73.9 at 100 K.Structural analysis by means of the multiple isomorphous replacement method is now in progress.Keywords
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