COMPARATIVE BINDING OF BEEF AND HUMAN INSULIN TO INSULIN ANTIBODIES PRODUCED IN MAN AND GUINEA PIGS*

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Abstract
The comparative effect of changing the concentration of beef or human insulin on the binding of beef insulin-I131 to insulin antibodies produced in the guinea pig or man was reinvestigated, using a technique involving the separation of bound from free insulin by preferential salt precipitation. Varying dilutions of 4 different preparations of crude human insulin produced changes in the binding of insulin-I131 to guinea pig antibody proportional to changes in concentration of crystalline beef insulin. Biological assay of the purest human insulin (Fisher insulin) was 4.08 [plus or minus] 19.8% U per mg. Immunological assay, using beef insulin as reference standard was 404 U per mg. SE = 0.43. It was concluded that extracted human and beef insulin cross react with guinea pig antibeef insulin in a proportional and nearly equivalent manner. Agreement between the immunoassay values for human insulin against beef insulin standards when either guinea pig or human antisera were used was demonstrable, suggesting that extracted human insulin binds almost as well to human antibeef-pork insulin antisera as does beef insulin.

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