Accumulation of nitrotyrosine on the SERCA2a isoform of SR Ca‐ATPase of rat skeletal muscle during aging: a peroxynitrite‐mediated process?
- 5 February 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 379 (3) , 286-290
- https://doi.org/10.1016/0014-5793(95)01530-2
Abstract
The SR Ca-ATPase in skeletal muscle SR vesicles isolated from young adult (5 months) and aged (28 months) rats was analyzed for nitrotyrosine. Only the SERCA2a isoform contained significant amounts with approximately one and four nitrotyrosine residues per young and old Ca-ATPase, respectively. The in vitro exposure of SR vesicles of young rats to peroxynitrite yielded selective nitration of the SERCA2a Ca-ATPase even in the presence of excess SERCA1a. No nitration was observed during the exposure of SR vesicles to nitric oxide in the presence of O2. These data suggest the in vivo presence of peroxynitrite in skeletal muscle. The greater nitrotyrosine content of SERCA2a from aged tissue imolies an age-associated increase in susceptibility to oxidation by this species.Keywords
This publication has 31 references indexed in Scilit:
- The reaction of NO· with O2·− and HO2·−: A pulse radiolysis studyFree Radical Biology & Medicine, 1995
- Nitration of Tyrosine by Hydrogen Peroxide and NitriteFree Radical Research, 1995
- Pulse Radiolysis of 2-Mercaptoethanol in Oxygenated Aqueous Solution. Generation and Reactions of the Thiylperoxyl RadicalThe Journal of Physical Chemistry, 1994
- Aromatic hydroxylation and nitration of phenylalanine and tyrosine by peroxynitriteFEBS Letters, 1994
- Cloned human brain nitric oxide synthase is highly expressed in skeletal muscleFEBS Letters, 1993
- Protein Oxidation and AgingScience, 1992
- Characterization and expression of the rat heart sarcoplasmic reticulum Ca2+‐ATPase mRNAFEBS Letters, 1989
- Nitrogen dioxide and related free radicals: electron-transfer reactions with organic compounds in solutions containing nitrite or nitrateJournal of the Chemical Society, Perkin Transactions 2, 1986
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The chemistry of pernitrites. Part I. Kinetics of decomposition of pernitrous acidJ. Chem. Soc. A, 1968