Influence of Blood Serum on Growth of Corynebacterium Bovis in Skimmilk and Trypticase Soy Broth

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Abstract
Stimulation of C bovis multiplication due to additions of various concentrations of bovine blood serum to skimmilk trypticase soy broth was studied. Raw serum stimulated multiplication in trypticase soy broth in concentrations as low as 0.001%. Stimulation was much greater at a concentration of 0.05% and increased slowly with additions of serum up to 5%. Whereas 0.1% raw serum was stimulatory when added to skimmilk, maximum stimulation occurred at the 4 and 5% concentrations, (bacterial numbers were more than 150 times those produced in skimmilk without added serum). Steaming of serum in broth caused more rapid multiplication of C. bovis than additions of raw serum to broth. Steaming of serum in skimmilk decreased the growth-stimulatory properties. Interactions between medium, serum treatment and test organism responsible for differences in growth response is unexplained.