The ht beta gene encodes a novel CACCC box-binding protein that regulates T-cell receptor gene expression.
- 1 September 1993
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 13 (9) , 5691-5701
- https://doi.org/10.1128/mcb.13.9.5691
Abstract
A gene encoding a novel CACCC box-binding protein that binds to the promoter region of the human T-cell receptor (TCR) V beta 8.1 gene and the mouse TCR alpha gene silencer has been cloned. This gene, termed ht beta, contains four zinc fingers of the class Cys2-X12-His2 that may be responsible for DNA binding and a highly negatively charged region that defines a putative transcriptional activation domain. Analysis of the expression of ht beta mRNA revealed similar expression levels and patterns in various cell lines. The bacterially expressed ht beta protein can bind to the CACCC box in both the human TCR V beta 8.1 gene promoter and the mouse TCR alpha gene silencer. The CACCC box is essential for efficient transcription of the V beta 8.1 promoter. Cotransfection with an ht beta expression plasmid and a reporter vector indicated that ht beta can activate human TCR V beta 8.1 gene transcription. ht beta also is able to counteract the silencing effect of the mouse TCR alpha gene silencer. The CACCC box has been found in almost all V beta 8.1 gene subfamily members and in both TCR alpha and beta gene enhancers in humans and mice. These results suggest that the CACCC box-binding protein may have an important regulatory function for TCR gene expression in alpha beta T cells versus gamma delta T cells.Keywords
This publication has 49 references indexed in Scilit:
- A T Cell-Specific Transcriptional Enhancer within the Human T Cell Receptor δ LocusScience, 1990
- αβ lineage-specific expression of the α T cell receptor gene by nearby silencersCell, 1989
- The same nuclear proteins bind the proximal CACCC box of the human β-globin promoter and a similar sequence in the enhancerBiochemical and Biophysical Research Communications, 1989
- Lineage-specific expression of a T cell receptor variable gene promoter controlled by upstream sequences.The Journal of Experimental Medicine, 1989
- How eukaryotic transcriptional activators workNature, 1988
- In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.Genes & Development, 1988
- Regulation of human interleukin-2 gene: Functional DNA sequences in the 5′ flanking region for the gene expression in activated T lymphocytesCell, 1986
- Limited diversity of the rearranged T-cell γ geneNature, 1985
- A third rearranged and expressed gene in a clone of cytotoxic T lymphocytesNature, 1984
- Complete primary structure of a heterodimeric T-cell receptor deduced from cDNA sequencesNature, 1984