Positional Cloning in Arabidopsis. Why It Feels Good to Have a Genome Initiative Working for You
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- 1 July 2000
- journal article
- review article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 123 (3) , 795-806
- https://doi.org/10.1104/pp.123.3.795
Abstract
Positional (or map-based) cloning techniques are widely used to identify the protein products of genes defined by mutation. In Arabidopsis the information generated by the Genome Initiative is giving this approach a decisive boost. A wealth of sequence polymorphisms and molecular markers is now available and can be exploited for fine mapping with technically simple and robust polymerase chain reaction-based methods. As a result it has become possible to complete positional cloning projects in a short time and with relatively little effort.Keywords
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