Synthesis, Conformation and Hydrolytic Stability of p1,p3−Dinucleoside Triphosphates Related to mRNA 5′-cap, and Comparative Kinetic Studies on their Nucleoside and Nucleoside Monophosphate Analogs
- 1 June 1990
- journal article
- research article
- Published by Taylor & Francis in Nucleosides, Nucleotides and Nucleic Acids
- Vol. 9 (4) , 599-618
- https://doi.org/10.1080/07328319008045191
Abstract
P1,P3−Dinucleoside triphosphates, N(5′)G(5′)G, have been prepared in which N is 7-Me-, 7-Et-, 7-Bn, N2, 7-diMe- or N2,N2, 7-triMe-guanosine. Conformations of the nucleoside moieties have been determined and compared with those of the corresponding nucleoside monophosphates. The hydrolytic stability of the 7-alkylguanine ring has been studied and the origin of the structural effects elucidated by comparative kinetic studies with monomeric nucleoside and nucleotide analogs. The mechanism of the alkaline decomposition has been established Or following the cleavage of 7-methylguanosine by 1H and 13C NMR spectroscopy.This publication has 29 references indexed in Scilit:
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