Elongases Synthesizing Very Long Chain Monounsaturated Fatty Acids in Developing Oilseeds and Their Solubilization
Open Access
- 1 August 1989
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 44 (7-8) , 629-634
- https://doi.org/10.1515/znc-1989-7-814
Abstract
Particulate (15,000 x g ) fractions from developing seeds of mustard (Sinapis alba), honesty (Lunaria annua), and nasturtium (Tropaeolum majus) synthesize radioactive very long chain (n9)-(Z)-monounsaturated fatty acids, e.g. gadoleic (20:1). erucic (22:1). and nervonic (24:1) acids from [1-14C ]oleoyl-CoA with malonyl-CoA or from [2-14C]m alonyl-CoA with oleoyl-CoA . Pyridine nucleotides are required for the elongation reactions. Both NADH and NADPH are equally effective for the formation of 20:1, but NADPH is the preferred reductant for the formation of 22:1, which suggest the existence of separate elongase systems. Detergents, such as Triton X-100 and octyl thioglucoside, stimulate the elongation at low concentrations, but they are inhibitory at high concentrations. Partial solubilization of the elongase system using these detergents has been achieved.Keywords
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