Optimization of bioassay method for the quantitative microbiological determination of amphotericin B
- 1 January 2010
- Vol. 53 (1) , 57-61
- https://doi.org/10.1111/j.1439-0507.2008.01660.x
Abstract
The aim of this study was to find the optimal bioassay parameters for the quantitative analysis of an amphotericin B nasal spray solution as the bioassay conditions recommended by the Ph. Eur. 6. were less sensitive and were only applicable for the measurement of a narrow concentration range, which makes the method unsuitable in case of a stability test. We evaluated five commonly used assay media with Candida albicans and Saccharomyces cerevisiae as test organisms. Our results showed that Mueller Hinton Agar supplemented with 2% glucose and 0.5 microg ml(-1) methylene blue inoculated with C. albicans gave the best bioassay circumstance as a wide concentration range (1.54-60.0 microg ml(-1) amphotericin B) could be measured and the inhibition zone borders were distinct and easy to read.Keywords
This publication has 7 references indexed in Scilit:
- Treatment of chronic rhinosinusitis with intranasal amphotericin B: A randomized, placebo-controlled, double-blind pilot trialPublished by Elsevier ,2004
- Evaluation of the Etest Method Using Mueller-Hinton Agar with Glucose and Methylene Blue for Determining Amphotericin B MICs for 4,936 Clinical Isolates of Candida SpeciesJournal of Clinical Microbiology, 2004
- Topical antifungal treatment of chronic rhinosinusitis with nasal polyps: A randomized, double-blind clinical trialJournal of Allergy and Clinical Immunology, 2004
- Stability of Mueller-Hinton Agar Supplemented with Glucose and Methylene Blue for Disk Diffusion Testing of Fluconazole and VoriconazoleJournal of Clinical Microbiology, 2004
- Effect of anti-fungal nasal lavage with amphotericin B on nasal polyposisThe Journal of Laryngology & Otology, 2002
- Analysis and assay of polyene antifungal antibiotics. A reviewThe Analyst, 1976
- Bioassay for Hamycin and Amphotericin B in Serum and Other Biological FluidsApplied Microbiology, 1969