ACTIVATION OF SCHRADAN BY MAMMALIAN TISSUE HOMOGENATES

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Abstract
It has been confirmed that the combined microsomal and soluble fractions of liver, in the presence of magnesium, nicotinamide, and DPN, can convert schradan to a potent anticholinesterase. DPN can be replaced by TPN, but not by ATP. DPNH can replace the DPN and soluble fraction. Catalase enhances the conversion but only when DPNH or a source of it is provided. Acetone powders of microsomes, suitably fortified, are almost ineffective in converting schradan. The pH optimum of the whole schradan-converting system is 8. 1. Other properties of the system are described. Certain extrahepatic tissues, especially lung, heart, and testis, can convert schradan. The livers of all species tested convert schradan. A peroxide-mediated mechanism of oxidation is proposed.