An antigen processing polymorphism revealed by HLA‐B8‐restricted cytotoxic T lymphocytes which does not correlate with TAP gene polymorphism

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Abstract
In previous studies of antigen presentation through HLA‐B27, we identified a healthy person whose lymphoblastoid cells do not present three B27‐restricted viral epitopes to specific cytotoxic T lymphocytes (CTL), despite adequate cell surface expression of HLA‐B2702 of normal sequence. Similar findings were observed in all members of his family sharing the HLA‐A3‐B2702 haplotype. The original donor, NW, carries HLA‐B8 on his other class I haplotype, which his daughter, HW, has inherited. We now report a failure to present an HLA‐B8‐restricted epitope from influenza nucleoprotein following viral infection of NW cells, although exogenous added peptide is still presented normally. However, cells from HW, which do not carry the A3‐B2702 haplotype, present the expected epitope after viral infection. Another B8‐restricted epitope, from human immunodeficiency virus‐gag, is presented equally well by both cell lines when infected with gag‐vaccinia. This antigen processing phenotype does not correlate with any of the known human TAP‐1 and TAP‐2 polymorphisms.