The Sialic Acids of Bovine and Equine Submaxillary Mucins.

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Abstract
The sialic acids of bovine submaxillary mucin (BSM) were separated and isolated by the aid of paper column chromatography. The predominant sialic acids in BSM are an N, O-diacetylneuraminic acid (with the O-acetyl in C-7 position) and an N-acetyl-di-O-acetylneuraminic acid (with one of the O-acetyls at C-7 and the other at C-8 or C-9). Small quantities of N-glycollylneuraminic acid and N-acetylneuraminic acid were also found, the latter possibly formed from the diacetylneuraminic acid during the isolation procedure. In addition there appeared small amounts of a fifth sialic acid which contained O-acetyl and was possibly a labile isomer of the N-acetyl-7-O-acetylneuraminic acid. The sialic acid of equine submaxillary mucin was isolated in pure crystaline form. It was identified as N-acetyl-4-O-acetylneuraminic acid.

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