Interference Due to Haemolysis in Routine Photometric Analysis—A Survey

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Abstract
To assess the affect of haemolysis on routine photometric methods used by participants in the Irish External Quality Assessment Scheme for Clinical Biochemistry (1EQAS) a sample was distributed with a supplied diluent containing haemoglobin. A sample of the same batch had previously been circulated as a normal distribution. Many analytes/methods were affected by the haemolysis. The range of values reported for each analyte was as follows. Albumin 34–48 g/L (baseline 37 g/L). Calcium 2.16–2.68 mmol/L (baseline 2.34 mmol/L). Urate 90–470 μmol/L (baseline 195 μmol/L). Creatinine 55–161 μmol/L (baseline 140 μmol/L). Glucose 4.1–5.6 mmol/L (baseline 4.8 mmol/L). Bilirubin 14–60 μmol/L (baseline 36 μmol/L). Urea 3.4–7.3 mmol/L (baseline 3.8 mmol/L). Iron 17.9–55.0 μmol/L (baseline 26.8 μmol/L). Inorganic phosphorus 0.22–2.41 mmol/L (baseline 1.59 mmol/L). Cholesterol 3.0–5.9 mmol/L (baseline 4.1 mmol/L). In some situations the use of an appropriate blank may solve the problem, but it must be remembered that interference caused by haemoglobin may be more complex than merely spectral.

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