Suppression of Costaining of Nonnervous Tissue in Protargol Technics

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Abstract
If, in the procedure of staining nerve fibers in mounted paraffin sections with Protargol according to Bodian, the reduction after toning with gold chloride is executed in a solution of 3–6 drops of aniline oil in 100 ml of 50% alcohol instead of in the prescribed oxalic acid solution, the selectivity of the staining of peripheral nerves is increased. This is effected by a reduction in the intensity of the staining of nonnervous tissue elements. However, at the same time the staining of nonnervous tissue is richer in details and consequently more satisfactory from a histological point of view than it is according to the original method of Bodian or the modification of this method by Ziesmer (1951).