Combined GTG-banding and nonradioactive in situ hybridization improves characterization of complex karyotypes
- 1 January 1990
- journal article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 54 (1-2) , 20-23
- https://doi.org/10.1159/000132947
Abstract
Nonradioactive in situ hybridization (ISH) using bio-tinylated centromere probes for chromosomes 1, 6, 7, 10, 16, 17, 18, and the X, respectively, was combined with GTG-banding to study cytogenetic changes in two different ovarian cancer cell lines. ISH was performed after GTG-banding on the same metaphase. The use of a low trypsin concentration (0.01%) in the banding procedure was essential for subsequent ISH. This combined approach allows the detection of subtle chromosomal rearrangements and appears to aid the identification of marker chromosomes.Keywords
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