Intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides
- 31 May 1985
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 54 (3) , 661-664
- https://doi.org/10.1128/jvi.54.3.661-664.1985
Abstract
The intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides was examined by polyacrylamide gel electrophoresis of [35S]methionine-labeled cell extracts under reducing conditions. All of the virion structural proteins were detected in cell extracts, including: L, 180,000 molecular weight (180K); P, 83K; HN, 69K; NP 66K; Fo, 60K; F1, 51R; and M, 38K. P and NP were phosphorylated. HN and F were glycosylated. The kinetics of intracellular viral protein synthesis did not detect any early or late proteins. Pulse-chase experiments failed to detect any precursor-product relationships. No nonstructural proteins were detected.This publication has 21 references indexed in Scilit:
- Resolution of two surface glycoproteins from human parainfluenza-3 virus by crossed immunoelectrophoresisJournal of Virological Methods, 1984
- Respiratory syncytial virus glycoproteinsVirology, 1983
- Natural history of parainfluenza virus infection in childhoodThe Journal of Pediatrics, 1982
- High Yield Growth and Purification of Human Parainfluenza Type 3 Virus and Initial Analysis of Viral Structural ProteinsJournal of General Virology, 1981
- Two disulfide-linked polypeptide chains constitute the active F protein of paramyxovirusesVirology, 1977
- A comparison of the polypeptides of four measles virus strainsVirology, 1977
- Methylmercury as a reversible denaturing agent for agarose gel electrophoresisAnalytical Biochemistry, 1976
- The synthesis of Sendai virus polypeptides in infected cellsVirology, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The proteins of the parainfluenza virus SV5Virology, 1969