Acid Hydrolysis of Erythropoietin.

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Abstract
Purified erythropoietin, obtained from the plasma of phenyl-hydrazine anemic rabbits by cellulose-ion exchange chromatography, has been submitted to mild acid hydrolysis. This mucoprotein has been shown for the first time to contain galactose, mannose and glucose. The hydrolytic removal of neuraminic acid is associated with a loss of erythropoietic activity as measured by reticulocyte response and Fe59 uptake by red cells in the rat.