IDENTIFICATION OF SUBSTANCE S IN HUMAN ADRENAL VENOUS BLOOD

Abstract
Use of the methylcyclohexane:dimethylformamide paper chromatographic solvent system resulted in the separation of 17-hydroxydesoxycorticosterone from corticosterone isolated from human adrenal venous blood. An unknown substance was also indicated. The amounts of 17-hydroxydesoxycorticosterone averaged 0.77 μg. per ml. of blood, whereas corticosterone was present in amounts averaging 2.8 μg. per ml. A comparison of the amounts of these substances with the amounts of hydrocortisone was also made.

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