DNA restriction endonuclease analysis for localization of human beta- and delta-globin genes on chromosome 11.
- 1 September 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (9) , 4563-4565
- https://doi.org/10.1073/pnas.76.9.4563
Abstract
DNA from a clone of a mouse-human hybrid that retained a human chromosome consisting of the major part of chromosome 11 and region q25-26-qter of the X chromosome was digested with various restriction endonucleases, subjected to electrophoresis in agarose gels, and transferred to nitrocellulose filters. The restriction digest pattern of the clone, when hybridized with a 32P-labeled plasmid fragment containing human .beta.-globin gene sequences, was a composite of the normal human and mouse (A9) patterns. When back-selected in 6-thioguanine to eliminate the 11 translocation chromosome, the hybrid cells showed only the A9 restriction pattern. The localization of .beta.- and .delta.-globin genes to human chromosome 11 is supported. The region 11q23-qter is excluded as the site for these genes.This publication has 22 references indexed in Scilit:
- The isolation and characterization of linked δ- and β-globin genes from a cloned library of human DNACell, 1978
- Polymorphism of DNA sequence adjacent to human beta-globin structural gene: relationship to sickle mutation.Proceedings of the National Academy of Sciences, 1978
- Analysis of the β-δ-globin gene loci in normal and hb lepore DNA: Direct determination of gene linkage and intergene distanceCell, 1978
- Chromosomal localization of human β globin gene on human chromosome 11 in somatic cell hybridsProceedings of the National Academy of Sciences, 1978
- Insertion of synthetic copies of human globin genes into bacterial plasmidsNucleic Acids Research, 1978
- A physical map of the DNA regions flanking the rabbit β-globin geneCell, 1977
- Localization of the human α-globin structural gene to chromosome 16 in somatic cell hybrids by molecular hybridization assayCell, 1977
- Gene mapping by fluorescent hybridizationCell Biology International Reports, 1977
- The Status of the Gene Map of the Human ChromosomesScience, 1977
- Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants.Proceedings of the National Academy of Sciences, 1977