Paper-chromatographic Studies on Flower Pigments of an Artificially Raised Amphidiploid and its Parent Plants

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Abstract
The materials used are Abelmoschus esculentus (2n=124), A. manihot (2n=68), F1 hybrids (2n=96) obtained by the cross A. esculentusX A. manihot and "Nori-Asa" (2n=192) which is an amphidipoid raised between the above 2 species. Paper-chromatography was used to determine the types of anthocyanins and their genetic behavior, using the flowers of the amphidiploid and its parents. The pigments in esculentus are ilicicyanin, chrysanthemin (or idaein) and cyanidin; in manihot they are delphinidin 3-glucoside (or empetrin) and delphinidin plus all components appearing in esculentus. Pigments in the F1 and the amphidiploid were found to be identical, in spite of an appreciable difference in their chromosomal constitution. The number of pigment components in these 2 plants was equal to that of manihot, but was apparently superior to that of esculentus plant.

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