Automatic Focusing of a Computer-Controlled Microscope
- 1 July 1975
- journal article
- Published by Institute of Electrical and Electronics Engineers (IEEE) in IEEE Transactions on Biomedical Engineering
- Vol. BME-22 (4) , 312-317
- https://doi.org/10.1109/tbme.1975.324450
Abstract
An on-line focusing procedure for a computer-controlled microscope employed in the measurement of human chromosome images is discussed. The method is based on an algorithm proposed by Mendelsohn and Mayall. A focusing accuracy of standard deviation less than 1/20th micron in the slide-objective distance has been achieved. Changes of the focus position with time, and for individual chromosomes within a metaphase, have been investigated. The method has proved insensitive to imperfections in the scanner photocathode. The method has also been used to determine the autofocusing accuracy of an analog metaphase-finding apparatus.Keywords
This publication has 3 references indexed in Scilit:
- Focus-Assist Device for a Flying-Spot MicroscopeIEEE Transactions on Biomedical Engineering, 1973
- Computer-Oriented analysis of human chromosomes—III. FocusComputers in Biology and Medicine, 1972
- Metaphase cell finding by machineCytogenetic and Genome Research, 1972