In vitro Method for Cultivating Eastern Equine Encephalomyelitis Virus In Teleost Embryos.

Publisher Website
Google Scholar
Abstract
Eastern equine encephalomyelitis (EEE), a human and animal viral pathogen, was cultivated in the fish, Gambusia. The sterile environment and simple medium routinely employed for propagating animal viruses, permitted development of the Gambusia eggs from early embryonic to free-swimming forms grossly similar to those seen at parturition. Concomitant with embryonic development, growth of the EEE virus was noted, potency for individual culture generations varying between 10-3.7-10-6.3. Double growth curves and other aspects of the work suggest we are dealing with a new facet of biological research.

This publication has 2 references indexed in Scilit: