Two Different Anti-Insulin Antibodies in Guinea Pig Antisera
Open Access
- 1 September 1962
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 89 (3) , 442-451
- https://doi.org/10.4049/jimmunol.89.3.442
Abstract
Summary: The antibodies in guinea pig anti-bovine insulin sera were fractionated by chromatography on DEAE cellulose. The anti-insulin antibody activity was found in two distinctly different fractions. Pooled antisera as well as antisera from individual animals contained both types of antibodies. However, the distribution of antibody activity in the two antibody fractions differed from serum to serum. The original antiserum and the two isolated antibody fractions showed very similar binding characteristics to crystalline bovine insulin. The antibodies differed in their electrophoretic mobility on starch: one migrated with the slow moving component of γ-globulin and the other with β2-globulin. Both antibodies were shown to have similar molecular size (probably of the order of 7 S globulins) by means of density gradient centrifugation.Keywords
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