A New Fluorometric Determination of Urinary 17-Hydroxy-Corticosteroids Using Glycinamide

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Abstract
We describe a new fluorimetric determination of urinary 17-hydroxycorticosteroids (17-OHCS) using the reaction of glycinamide with compounds carrying dihydroxyacetone side chain in borate buffer (pH 9.8). The fluorescent compound has excitation and emission maxima at 325 and 385 nm, respectively. The method includes enzymatic hydrolysis with β-glucuronidase and extraction with methylene chloride and generation of fluorescence in weakly alkaline borate buffer. Specificity of the reaction was examined and results were compared with those of an accepted method based on the Porter-Silber reaction. Results were satisfactory for measuring urinary 17-OHCS.