Serum Lipase Determination with an Olive Oil Substrate Using a Three-Hour Incubation Period
- 1 October 1962
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 8 (5) , 509-519
- https://doi.org/10.1093/clinchem/8.5.509
Abstract
The effect of various emulsifying agents, pH, buffers, and accelerators on the Cherry-Crandall procedure for determining serum lipase was studied. A method is proposed which uses a 3-hour incubation period. Olive oil emulsified with 7% acacia is the substrate. This is buffered with tris buffer, pH 8.0; magnesium acetate and EDTA are added as accelerators. Other conditions which result in an increased rate of hydrolysis are discussed.Keywords
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