DNA analysis based on physical manipulation
- 7 November 2002
- conference paper
- Published by Institute of Electrical and Electronics Engineers (IEEE)
Abstract
A novel DNA analysis method based on physical molecular manipulation is proposed and experimentally demonstrated. On a glass substrate is deposited a sacrificial layer, carrier layer and a pair of electrodes, onto which the DNA solution is fed. By applying high frequency voltage, the DNA is stretched straight and aligned, with one of its molecular end anchored on the electrode edge, and immobilized onto the carrier layer. Then using an AFM stylus as a knife, a portion of the DNA together with the carrier layer is cut, and by dissolving the sacrificial layer, recovered onto a membrane filter. Successful recovery is demonstrated using PCR.Keywords
This publication has 5 references indexed in Scilit:
- Molecular surgery of DNA based on electrostatic micromanipulationPublished by Institute of Electrical and Electronics Engineers (IEEE) ,2002
- Electromechanics of ParticlesPublished by Cambridge University Press (CUP) ,1995
- Applications of electrostatic stretch-and-positioning of DNAIEEE Transactions on Industry Applications, 1995
- Alignment and Sensitive Detection of DNA by a Moving InterfaceScience, 1994
- Electrostatic manipulation of DNA in microfabricated structuresIEEE Transactions on Industry Applications, 1990