The Fine Structures in Melting Curves of Deoxyribonucleic Acids of Bacteriophage Lambda. I

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Abstract
Remarkable fine structures have been observed in the melting and renaturation curves of phage lambda DNA with our automatic recording apparatus of heating-cooling curves of UV absorbance. The fine structures are thought to originate from the unusual internal heterogeneity of the base sequence of the DNA molecule of bacteriophage lambda. A new method for the analysis of the fine structures in the melting and renaturation curves is described. Using this method, the molecular process of melting of phage lambda DNA and the detail of the molecular ensemble at an intermediate stage of denaturation are analyzed.