Linear Ferguson plots of polystyrene sulfate size standards for the quantitative agarose gel electrophoresis of subcellular particles
- 1 January 1988
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 9 (6) , 243-255
- https://doi.org/10.1002/elps.1150090602
Abstract
Accurately standardized commercial polystyrene sulfate particles in agarose gel electrophoresis yield linear Ferguson plots at pH 7.4 over a gel concentration range up to 0.9% agarose which do not exhibit any significant sigmoidal curve elements, using either a discontinuous buffer system or a continuous buffer. Ferguson plots of these standard-sized particles were evaluated using alternatively a linear or convex model, by means of a newly developed set of programs (to be used in conjunction with program M-LAB) which (i) is sufficiently user-friendly to allow for quantitative agarose gel electrophoresis of subcellular-sized spherical particles based on their convex Ferguson plots with the same operational simplicity previously available for linear Ferguson plots only; (ii) simultaneously and interactively analyzes the Ferguson plots of all particles under consideration on the basis of an extended Ogston model.This publication has 19 references indexed in Scilit:
- Discontinuous buffer systems optimized for the agarose gel electrophoresis of subcellular particlesElectrophoresis, 1988
- Gel electrophoresis of intact subcellular particlesJournal of Chromatography B: Biomedical Sciences and Applications, 1987
- Agarose gel electrophoresis of bacteriophages and related particlesJournal of Chromatography B: Biomedical Sciences and Applications, 1987
- Computer simulation of the variable agarose fiber dimensions on the basis of mobility data derived from gel electrophoresis and using the Ogston theoryAnalytical Biochemistry, 1987
- Polystyrene latex particles as size standards in quantitative agarose gel electrophoresis: Application to three plant virusesElectrophoresis, 1987
- Moving boundary electrophoresis on agarose gel of plant viruses and polystyrene microspheresElectrophoresis, 1987
- Characterization of subcellular particles by size, charge and apparent compressibility on the basis of mobility in agarose gel electrophoresis: Procedures of computer simulationElectrophoresis, 1987
- [8] Use of gel electrophoresis to characterize multimolecular aggregatesPublished by Elsevier ,1986
- Analysis of convex Ferguson plots in agarose gel electrophoresis by empirical computer modelingElectrophoresis, 1986
- Agarose gel electrophoresis of bacteriophages and related particles. III. Dependence of gel sieving on the agarose preparationElectrophoresis, 1983