Direct selection of antibodies that coordinate metals from semisynthetic combinatorial libraries.
- 15 July 1993
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 90 (14) , 6385-6389
- https://doi.org/10.1073/pnas.90.14.6385
Abstract
An iterative strategy for the selection of catalytic metalloantibodies is described. The first stage of this strategy is validated by the selection of semisynthetic antibodies that coordinate a variety of different metal ions and the metal oxide magnetite. These results have implications not only for the development of catalytic metalloantibodies but also for the development of reagents for magnetic resonance imaging, delivery of radioisotopes, and purification of recombinant proteins.Keywords
This publication has 21 references indexed in Scilit:
- Applications of bacterial magnetsPublished by Elsevier ,2003
- Magnetoferritin: in Vitro Synthesis of a Novel Magnetic ProteinScience, 1992
- Metal-binding sites in proteinsCurrent Opinion in Biotechnology, 1991
- Grafting of a calcium-binding loop of thermolysin to Bacillus subtilis neutral proteaseBiochemistry, 1991
- Regulation of serine protease activity by an engineered metal switchBiochemistry, 1990
- MetalloantibodiesScience, 1990
- Zinc coordination, function, and structure of zinc enzymes and other proteinsBiochemistry, 1990
- Chiral Metal Complexes as Discriminating Molecular CatalystsScience, 1990
- Canonical structures for the hypervariable regions of immunoglobulinsJournal of Molecular Biology, 1987
- Pb(II)-catalysed cleavage of the sugar–phosphate backbone of yeast tRNAPhe—implications for lead toxicity and self-splicing RNANature, 1983