Dinucleoside pyrophosphate are substrates for T4-induced RNA ligase.
- 1 November 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (11) , 4839-4842
- https://doi.org/10.1073/pnas.74.11.4839
Abstract
RNA ligase isolated from bacteriophage T4-infected Escherichia coli will utilize a number of different compounds with the general structure Ado-5''PP-X [adenylated donor oligonucleotide pyrophosphates] as substrates in an ATP-independent reaction. The P-X portions of these molecules are transferred to the 3''-hydroxyl of an oligoribonucleotide to form a phosphodiester bond, and the AMP portion is released. AMP, CMP, GMP, UMP, dTMP, NMN, .alpha.NMN, reduced NMN, FMN, Rib-5P [ribulose 5-phosphates], phosphopantetheine and cyanoethylphosphate all were added to [Cyd[cytidine]-3H](Ap)3C [acceptor] from their corresponding AMP adducts. Contrary to the relative lack of specificity of RNA ligase for the P-X group added, the failure of NADP+, deamino-NAD+, .epsilon.NCD+ [nicotinamide 3,N4-ethenocytosine dinucleotide], .epsilon.NAD+ [nicotinamide 1-N6-ethenoadenine dinucleotide] and CoA to react indicates that the enzyme shows a high degree of selectivity for the AMP portion of the substrate. The diversity of chemical groups that can be efficiently added suggests that this reaction of RNA ligase will be useful for the modification of the 3'' ends of RNA molecules.This publication has 13 references indexed in Scilit:
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