The influence of the Klebsiella pneumoniae regulatory gene nifL upon the transcriptional activator protein NifA
- 1 August 1990
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 4 (8) , 1253-1258
- https://doi.org/10.1111/j.1365-2958.1990.tb00704.x
Abstract
The influence of the Klebsiella pneumoniae nifL gene product upon the interaction of the transcriptional activator protein NifA with the nifH promoter has been examined using in vivo dimethylsulphate ‘footprinting’. Binding of NifA to the upstream activator sequence (UAS) of the nifH promoter in the presence of the NifL protein was observed under nitrogen‐limiting growth conditions. Growth in the presence of NH+4 or addition of NH+4 to nitrogen‐limited cells diminished the interaction of NifA with the UAS when NifL was present. Repression of nif transcription by NifL may therefore involve an interaction between NifL and NifA which reduces the affinity of NifA for the UAS.This publication has 37 references indexed in Scilit:
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