Expression and Phosphorylation of BiP/GRP78, a Molecular Chaperone in the Endoplasmic Reticulum, during the Differentiation of a Mouse Myeloblastic Cell Line.

1 January 1995

journal article
Published by Japan Society for Cell Biology in Cell Structure and Function

Vol. 20 (1) , 33-39
https://doi.org/10.1247/csf.20.33

Abstract

To determine the functional significance of endoplasmic reticulum chaperones in hematopoietic cells, we analyzed the expression and post-translational modification of BiP/GRP78 and GRP94 as well as the cytoplasmic chaperones HSP70 and HSC70 during the differentiation of a mouse myeloid leukemia cell line, M1. The amounts of BiP/GRP78 and GRP94increased several-fold when M1 cells were induced to differentiate into macrophage-like cells by treatment with interleukin-6 (IL-6). Synthesis began to increase at 4 hr after IL-6 treatment. The phosphorylated form of BiP/GRP78 increased during the later stages of differentiation. These data suggested that the chaperone activity of BiP/GRP78 and GRP94 may be needed for differentiated macrophage-like cells or for the differentiation event itself, and that functionally different BiP/GRP78 accumulate during the differentiation of M1 cells.

Keywords

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