Genetic defect of the sodium pump-defective mutant nap-1 from the marine Vibrio alginolyticus
Open Access
- 1 January 1997
- journal article
- Published by Wiley in IUBMB Life
- Vol. 41 (1) , 41-47
- https://doi.org/10.1080/15216549700201041
Abstract
The marine bacterium, Vibrio alginolyticus, has a respiratory chain‐linked Na+‐translocating NADH‐quinone reductase (NQR). Among several mutant cells defective in Na+ pump activity, Nap1 was a very stable mutant and a spontaneous revertant could not be isolated from Nap1. Using genetic irformation from the recently sequenced nqr operon, the genetic defects in Nap1 were examined, and the sodium pump‐defective mutant Napl was found to be caused by the insertion of a 1.2 kbp DNA fragment into the C‐terminal region of nqr6 gene.Keywords
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