CHARACTERIZATION OF BETA-D-N-ACETYLHEXOSAMINIDASE ISOENZYMES IN MAN CHINESE HAMSTER SOMATIC-CELL HYBRIDS

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 29  (1) , 14-23
Abstract
A series of man-Chinese hamster hybrids were investigated with the use of an anti-Chinese hamster hexosaminidase serum, a specific anti-human hex A serum and an anti-human hex B serum. The expression of human hex A was dependent on the presence of hex B. A heteropolymeric molecule is formed independently of hex B, which consists of Chinese hamster and specific hex A moieties. It has an electrophoretic mobility nearly identical to hex A. A relationship between the absence and presence of the heteropolymeric molecule, mannosephosphate isomerase (MPI), and pyruvate kinase (PK-3), assigned to chromosome 15, was established. With respect to the 2 locus subunit model, the gene coding for the .alpha. subunit, specific for hex A, was localized on chromosome 15.