Application of NMR screening techniques for observing ligand binding with a protein receptor
- 8 October 2004
- journal article
- research article
- Published by Wiley in Magnetic Resonance in Chemistry
- Vol. 43 (1) , 69-72
- https://doi.org/10.1002/mrc.1492
Abstract
Water ligand observed via gradient spectroscopy (WaterLOGSY), saturation transfer difference and NOE pumping NMR techniques were used to identify ligand binding with a receptor. Although these experiments were originally designed to observe ligands in complexes, their application is limited by the affinity of ligands towards target molecules. Here the improved WaterLOGSY pulse sequence was developed by incorporating the double pulsed field gradient spin-echo and gradient-tailored excitation WATERGATE sequences. The efficiency of these ligand-observed NMR screening techniques was investigated using the ribonuclease T1-inhibitor system. Copyright © 2004 John Wiley & Sons, Ltd.Keywords
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