Sporulation of Saccharomyces cerevisiae in the Absence of a Functional Mitochondrial Genome
- 1 January 1974
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 117 (1) , 80-88
- https://doi.org/10.1128/jb.117.1.80-88.1974
Abstract
The role of the mitochondrial system during sporulation of Saccharomyces cerevisiae was studied. Addition of ethidium bromide (EthBr) to cells growing in acetate medium resulted in the quantitative (>98%) conversion of the culture to the petite genotype in one generation. The cells were respiratory active (derepressed) but contained no mitochondrial deoxyribonucleic acid (mtDNA) as demonstrated by analytical ultracentrifugation in CsCl. When transferred to acetate sporulation medium, the culture sporulated. Ascus production was only slightly below that of the control culture. Synthesis of mtDNA occurred during sporulation in the control but not in the EthBr-treated culture. Mitochondrial protein synthesis was virtually eliminated in the EthBr-treated culture. Therefore, completely derepressed cells can sporulate without a functional mitochondrial genetic system. When partially repressed cells were treated with EthBr, no ascus formation was observed after transfer to sporulation medium. Control cultures underwent respiratory adaptation in sporulation medium and then sporulated. Extensive derepression of the respiratory system is thus required for sporulation, and this adaptation is dependent on a functional mitochondrial system. Our results suggest that once the cells are fully derepressed no mitochondrial genetic information has to be expressed during meiosis and ascus formation.Keywords
This publication has 22 references indexed in Scilit:
- Sporulation in Saccharomyces cerevisiae: Premeiotic DNA synthesis, readiness and commitmentExperimental Cell Research, 1972
- The genetic control of sporulation inSaccharomycesMolecular Genetics and Genomics, 1972
- Biogenesis of mitochondria: XXI. Studies on the nature of the mitochondrial genome in yeast: The degenerative effects of ethidium bromide on mitochondrial genetic information in a respiratory competent strainJournal of Molecular Biology, 1972
- Chapter 2 Regulation of Sporulation in YeastPublished by Elsevier ,1972
- Effects of mutagenic treatment by ethidium bromide on cellular and mitochondrial phenotypeArchives of Biochemistry and Biophysics, 1972
- Erythromycin inhibition of sporulation inSaccharomyces cerevisiaeCellular and Molecular Life Sciences, 1971
- The petite mutation in YeastJournal of Molecular Biology, 1970
- Protein synthesis by yeast promitochondria in vivoBiochemical and Biophysical Research Communications, 1969
- Ethidium bromide induced mutation of yeast mitochondria: Complete transformation of cells into respiratory deficient non-chromosomal “petites”Biochemical and Biophysical Research Communications, 1968
- THE METABOLISM OF YEAST SPORULATION: I. EFFECT OF CERTAIN METABOLITES AND INHIBITORSCanadian Journal of Microbiology, 1956