High-speed Liquid Chromatographic Determination of Colouring Matters in Capsules

Publisher Website
Google Scholar
Abstract
A rapid estimation procedure using a high-speed liquid chromatography is described for determination of ammaranth, tatrazine, Sunset Yellow FCF, Erythrosine, Phloxine B, Rose Bengal, and Acid Red. Separation can be achieved within 15 min employing a 50 cm column packed with Permaphase ODS, using gradient from 0.05% cetyltrimethylammonium bromide in 20% MeOH to MeOH as an eluant with a Shimadzu Du Pont LC-2 liquid chromatograph. Accuracy in this method was found to be less than ±1.5%. The limit of detection was several nanograms. Colouring matter in gelatin capsules were examined by this analytical method. One empty capsule is dissolved in 5 ml of 0.1 N NaOH and the mixture is passed through a column (1×5 cm) containing active charcoal. Gelatin is removed by passing 100 ml of H2O through the column. The colouring matter are then eluted with 50% pyridine, the eluate is evaporated to dryness, the residure is dissolved in a small amount of H2O and examined by this method.

This publication has 0 references indexed in Scilit: