Isolation of Highly Purified and Stable Preparations of c-src and v-src Kinase
- 1 January 1991
- journal article
- research article
- Published by S. Karger AG in Cellular Physiology and Biochemistry
- Vol. 1 (1) , 24-30
- https://doi.org/10.1159/000154590
Abstract
We describe here a simple method of purification of src kinase from insect cells infected with a baculovirus expressing c-src kinase or v-src kinase. After two affinity columns prepared with random synthetic polypeptides, highly purified protein tyrosine kinases were obtained that remained active for many months of storage at –70°C.Keywords
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