Characteristics of the cell surface antigen, p72, associated with a variety of human tumours and mitogen‐stimulated T‐lymphoblasts

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Abstract
The monoclonal antibody 791T/36, prepared against a human osteogenic sarcoma cell line, 791T, reacts with a variety of human tumours and also mitogen‐stimulated PBMN cells. The target antigen as expressed upon 791T cells is a monomeric plasma membrane‐associated glycoprotein with an apparent M r of 72000. By quantitative flow cytofluorimetry, approx. 105 antibody molecules bound per cell to T‐lymphoblasts induced with PHA or Con A, whereas only a few thousand antibody molecules bound per cell to unstimulated cells, so that the antigen may be classified as a lymphocyte activation antigen. On lymphoblasts, the 791T/36 again reacted with a protein with an apparent M r of 72000. This antigen therefore has a dual role as a tumour marker and lymphocyte activation antigen which may be implicated in the regulation of cell proliferation.